Publication Date: 2022/08/13
Abstract: In this study, the nodal explants collected from the healthy shoots were cultured on the basal MS medium supplemented with 3mg/lit of BAP (6-Benzyl Amino Purine). In the earlier study, several researchers reported that the concentration of 3mg/lit is optimum for the growth and development of shoot tip in rose by the duration of 1month. The different stage of shoot development was observed from the third day to one month interval. It revealed that the callus initiation starts from the 10th day and auxillary shoot initiation from 2 weeks, and subsequent growth and development was observed from the third week and one month interval respectively. Further the developed healthy shoot culture will be maintained and will be treated with NAA at different concentration for root initiation. Finally, the optimum concentration of NAA will be fixed for better root initiation and development
Keywords: Micropropagation of Rose.
DOI: https://doi.org/10.5281/zenodo.6987919
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT22JUL1139.pdf
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