Publication Date: 2020/07/01
Abstract: Ca2+ sequestration and its homeostasis is disrupted when mitochondrial membrane permeabilises to form a large opening - the mitochondrial permeability transition pore (MPTP). The MPTP formation is common during aging and age related pathologies, activating cell death pathways to avoid unhealthy consequences and malignancies in the brain tissue. Several studies have identified the participation of Cyclophilin-D (Cyp-D) and adenine nucleotide translocase(ANT) in forming MPTP. However, Ca2+ is known to participate significantly in MPTP induction, although its concentration and time dependent permeabilization mechanisms are still elusive. In this work, we have focused on the contribution of Ca2+ participation, its concentration and time taken to permeabilze the mitochondrial inner membrane as a measure of light scattering at 540 ηM. We have observed that MPTP formation is increased in mitochondria isolated from aged rats in comparison to young adult and neonatal rats. The cyclosporin A application blocks the cyclophilin interaction thus avoiding MPTP formation confirming the crucial role of Ca2+ inducted MPTP opening. A 100 µM Ca2+ incubation for 15 minutes allowed the 50% probability of formation of MPTP in mitochondria isolated from all age groups. Thus, alleviating its role in aging and neurodegeneration.
Keywords: Mitochondria;Ca2+ ;Aging;Mitochondrial Permeability Transition Pore (MPTP).
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT20JUN323.pdf
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