Publication Date: 2024/02/09
Abstract: It is a vital requirement to improve analytical methods to identify individuals of drugs of abuse. Analytical method was developed to identify methadone in urine specimens using solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). GracepureTM C18 column was used for SPE and GC-MS was operated in electron impact ionization full scan mode. Hexadecane was used as an internal standard. The SPE-GC-MS method was validated for urine spiked with methadone with a concentration range of 0.010 to 0.050 mg mL-1 and acceptable linearity (R2 = 0.9905 ± 0.0052) was observed. The LOD and LOQ values for urine spiked with methadone obtained in this research were 0.193 ± 0.008 μg mL-1 and 0.607 ± 0.023 μg mL-1 respectively. The developed method was convenient for the extraction and quantification of methadone in urine.
Keywords: Methadone, Hexadecane, Gas Chromatography-Mass Spectrometry, Solid Phase Extraction, C18 Column.
DOI: https://doi.org/10.5281/zenodo.10638368
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT24FEB168.pdf
REFERENCES