Evaluation of the Immunodot Technique Compared to the Elisa Technique for the Detection of IgA Antibodies to Tissue Transglutaminase

Oussama Aazzane; Hasnaa Bazhar; Hassan Fellah1

1

Publication Date: 2025/01/02

Abstract: Celiac disease (CD) is a chronic autoimmune enteropathy characterized by intestinal villous atrophy secondary to gluten ingestion. In the biological diagnosis of CD, serology includes, among other tests, the search for IgA anti-tissue transglutaminase antibodies (IgA anti-tTG). Various techniques are employed, including ELISA and Immunodot. The objective of this work is to evaluate the diagnostic performance of the Immunodot test compared to the ELISA test for detecting IgA anti- tTG antibodies in the serum of patients with CD and controls. This is a comparative study of the Immunodot test against the ELISA test for the detection of IgA anti- tTG antibodies. The antibody dosage was performed on 96 samples belonging to 49 patients with CD and 47 healthy subjects. All samples were tested using the Immunodot test (DotDiver CeliAK IgA) and the ELISA test (Anti-tissue Transglutaminase Elisa (IgA) Test instruction). The comparison of the Immunodot test to the ELISA test revealed a sensitivity of 59% and a negative predictive value (NPV) of 73%.

Keywords: Celiac Disease, Anti-TG Antibodies, Immunodot, Elisa, IgA.

DOI: https://doi.org/10.5281/zenodo.14576664

PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT24DEC1456.pdf

REFERENCES

  1. Tasuku T,Kenji I. Celiac Disease Genetics, Pathogenesis, and Standard Therapy for Japanese Patients. International Journal of Molecular Sciences. 2023 Jan 20 ;24(3) :2075. doi: 10.3390/ijms24032075.
  2. KHATER S, CELLIER C. Celiac disease: diagnosis and treatment. Journal of Medical Biology. 2020.
  3. National Institutes of Health consensus development conference statement on celiac disease, June 28–30, 2004. Gastroenterology. 2005; 128(4 Suppl 1):S1–9. doi: 10.1053/j.gastro.2005.02.007.
  4. Fasano A, Catassi C. Clinical practice. Celiac disease. The New England Journal of Medicine. 2012 ; 367(25) :2419–26. doi.org/10.1056/NEJMcp1113994.
  5. Kelly CP, Bai JC, Liu E, Leffler DA. Advances in diagnosis and management of celiac disease. Gastroenterology. 2015; 148(6):1175–86. doi: 10.1053/j.gastro.2015.01.044.
  6. Malamut G, Cellier C. Celiac disease. EMC. 2016.  doi: 10.1016/S1634-6939(16)60111-4.
  7. Husby S, Koletzko S, Korponay-Szabó I, et al. European Society Paediatric Gastroenterology, Hepatology and Nutrition Guidelines for Diagnosing Coeliac Disease 2020. Journal of Pediatric Gastroenterology and Nutrition. 2020 Jan;70(1):141-156. doi: 10.1097/MPG.0000000000002497.
  8. Khadiry B, Fellah H, et al. Evaluation of the ELISA technique compared to Immunodot in the screening of extractable nuclear antigens antibodies Sm, SSA and SSB. International Journal of Innovation and Applied Studies. 2022, May, pp. 493-501.
  9. Ousmane S, El Fakir S, El Rhazi K, et al. Estimating the roc curve. Moroccan Journal of Public Health. 2015, vol 2, n° 2.  
  10. Schaefer L, Dyke  J , Meglio F, et al. Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMx automated analyzer. Journal of Clinical Microbiology.1989 Nov; 27(11):2410-3. doi: 10.1128/jcm.27.11.2410-2413.1989.