Publication Date: 2023/12/25
Abstract: This study evaluated the type of mutation associated with SNPs of black skinned and white skinned snails (Archachatina marginata) in the rainforest zone of Nigeria. Fourty eight (48) Archachatina marginata snails were assigned to two treatments using completely randomized design. The two treatments consisted of twenty four (24) white skinned ecotypes and twenty four(24) black skinned ecotypes with 12 replicates each. These snails were taken to laboratory to conduct DNA analysis to evaluate the mutation type that is associated with the SNPs of the two snail ectotypes. The quality of DNA was assessed using gel electrophoresis. Data collected was subjected to statistical analysis using Mega 7.0, DNAsp version 5 and GENPOP software packages. Mutation result obtained in this study revealed that both types undergo both substitution and deletion with deletion being predominant in the black and substitution in the white skinned A. marginata. It was also observed that the codons mostly affected by deletion are the nucleotides coding for amino acids phenyl alanine and alanine. These amino acids are responsible for growth, immunity and skin colouration. Since the immune-related genes are absent in black people, the high rate of deletion that black experience may be the cause of their high mortality rate. Self-fertilization, a high degree of inbreeding, and restricted gene flow all contribute to the high rate of deletion. Thus, it would be beneficial to promote the crossbreeding of A. marginata snails with black and white skin.
Keywords: Mutation, Evaluation, Substitution, Deletion.
DOI: https://doi.org/10.5281/zenodo.10473256
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT23DEC536.pdf
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