Publication Date: 2023/07/15
Abstract: The beneficial effects of bioremediation with oil-degrading microorganism, Pseudomonas aeruginosa and Bacillus subtilis, on tomato seedlings growth in oil- contaminated soil was investigated. The effect of bioremediation was examined with Pseudomonas aeruginosa and Bacillus subtilis on phytotoxicity, as measured by tomato seed growth. Tomato growth parameters (leaf length, height, seed germination, chlorosis, stunted growth) were evaluated daily for 15days.Some qualitative morphological observations made on the seedlings were also observed for seedlings. In the untreated control as well as the treated experimental samples (Pseudomonas aeruginosa and Bacillus subtilis), sprouting of the seeds were rapid and completed in a few days, the seedlings showed steady growth and morphologically normal. In the control soils however, sprouting was sluggish, and growth was stunted, yellowing of the leaves was observed (chlorosis); a number of the seedlings were folded at the tips. These were indications of phytotoxicity resulting from oil pollution. The results of the present research have proven the bioremediation potential of bacterial isolates, for crude oil contamination which also showed significant growth potential for the tomato seeds.
Keywords: Phytotoxicity, Pollution, Crude Oil, Pseudomonas aeruginosa, Tomatoes.
DOI: https://doi.org/10.5281/zenodo.8149736
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT23JUN974.pdf
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