Publication Date: 2023/12/08
Abstract: Chikungunya virus was purified with Cesium chloride (CsCl) density gradients by ultracentrifugation from the cell culture supernatant of infected Vero cell. The purification process was done by using combinations of CsCl and PBS (Phosphate buffer saline) and the virus band was found at a density of 1.00 g/ml after centrifugation for 3 hr of ultracentrifugation. The authenticity of the purified Chikungunya virus was confirmed by SDS PAGE. The Purification process has immense application and is useful in making large quantity of purified Chikungunya virus with high titer values for its characterization.
Keywords: Chikungunya Virus, Centrifugation, Vero Cells, CsCl, TFF, Antigen.
DOI: https://doi.org/10.5281/zenodo.10298353
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT23DEC021.pdf
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