Publication Date: 2023/12/06
Abstract: The bacteria community in the rhizosphere region is critical for the growth of plants and the formation of soil organic matter. This study focuses on the identification of rhizospheric soil bacteria from rice varieties grown under greenhouse environment. Samples of Oryza sativa and Oryza glabberima were collected from the National Cereals Research Institute (NCRI) Umuahia and Rice mill at Kpirikpiri Ebonyi State. Oryza sativa grew more rapidly than Oryza glabberima because of the variations in rice species. The rice seeds were planted in nursery and transplanted in buckets under greenhouse conditions (75% humidity and at room temperature 25°C). The adhering soil samples from the rhizosphere region were gotten from the roots and 1g of soil weighed aseptically and dissolve in sterile distilled water. An aliquot was picked for serial dilution. The serially diluted aliquot was inoculated on nutrient agar and incubated. Individual colonies were subcultured. Microbiological, biochemical and sugar fermentation tests were able to classify the isolates from Oryza sativa into Azotobacter spp., and Bacillus spp., and Oryza glabberima into Bacillus spp and Pseudomonas spp. respectively. This study showed that there are a lot of soil bacteria in the rhizospheric region of Oryza sativa than Oryza glabberima because it is an improved variety and enhance the utilization of available nutrients and minerals by microbial interaction which enable rapid growth of the plant, therefore, Oryza sativa should be cultivated more for high yield and large-scale agricultural production.
Keywords: Oryza Sativa, Oryza Glabberima, Rhizosphere, Bacteria, Greenhouse, Nerica5, M306.
DOI: https://doi.org/10.5281/zenodo.10426965
PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT23NOV1638.pdf
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