Assessment of the Histochemical Occurrence of Ferric Iron and Calcium Ion in Liver Biopsies and their Correlation with Liver Disease: A Retrospective Study

Hassan Musa Chiwar; Agyigra Aksavdwa Isaac; Modu Ahmed; Hayatuddeen Umar Bulama; Fatima Aliyu Chiroma; Umar Musa Ahmad; Fanta Abba Kolo1

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Publication Date: 2023/07/17

Abstract: Liver disease is a major global health concern, with iron overload and calcium dysregulation implicated in the pathogenesis of several liver diseases. This retrospective case-control study aimed to determine the presence or absence of ferric iron and calcium ion histologically in formalin-fixed paraffin-embedded liver biopsies diagnosed with various liver diseases. The study found that the staining techniques employed in this study were reliable and effective for detecting ferric iron and calcium ion in FFPE liver biopsies, but no significant presence of these ions was detected in any of the samples. Therefore, the study did not find evidence to support the hypothesis that patients with liver disease should take iron and/or calcium supplements. These findings highlight the need for further research to fully understand the role of iron and calcium in liver disease.

Keywords: liver disease, histological examination, liver biopsies, Prussian blue stain, retrospective case-control study.

DOI: https://doi.org/10.5281/zenodo.8153322

PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT23JUN1907.pdf

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