Antibacterial Activity of Senggani (Melastoma malabathricum L.) Leaves Extract against Pseudomonas aeruginosa and Staphylococcus aureus

Regina Septiana Barung; Boy Rahardjo Sidharta; P. Kianto Atmodjo1

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Publication Date: 2024/01/09

Abstract: Ethanolic extract of senggani (Melastoma malabathricum L.) leaf contains secondary metabolites such as flavonoids, tannins, steroids, alkaloids, saponins, and triterpenoids. Flavonoids which are dominant among other compounds have shown antibacterial activity, especially in Pseudomonas aeruginosa and Staphylococcus aureus. The research aims to determine the inhibition zone and minimum inhibition concentration (MIC) of the leaf extract against P. aeruginosa and S. aureus. The leaf extract is done by maceration utilizing 70 % ethanol (pa) and variation of concentrations viz. 5, 10, and 15 % were treated to P. aeruginosa and S. aureus. The positive control is ampicillin, and the negative control is the solvent (ethanol 70 %). The results show that the leaf extract at 15% shows the highest inhibition zone against P. aeruginosa and S. aureus at 0.63±0.18 and 1.48±0.47 cm2 , respectively. The MIC for P. aeruginosa is 15% and for S. aureus is 10%.

Keywords: Melastoma malabathricum, antibacterial activity, MIC, skin infection

DOI: https://doi.org/10.5281/zenodo.10473992

PDF: https://ijirst.demo4.arinfotech.co/assets/upload/files/IJISRT23DEC1559.pdf

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